Ratiometric electrochemiluminescent aptasensor array for antibiotic based on internal standard method and spatial-resolved technique

- Ratiometric ECL aptasensor strategy based on the ratio of working signal to internal standard signal was designed.
- L-Au NPs as ECL working tags and CdS QDs as internal standard tags were respectively modified on two spatial resolved electrodes.
- The CAP aptamer connection with CA was used as quenching probes of L-Au NPs.
- The CAP combination with quenching probes could restore ECL of L-Au NPs.
- The ratio of two ECL intensities was used for specific assay of CAP.