کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7144984 | 1462068 | 2016 | 36 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Influence of alkane chain lengths and head groups on tethering of liposome-gold nanoparticle on gold surface for electrochemical DNA sensing and gene delivery
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Influence of alkane chain lengths and head groups on tethering of liposome-gold nanoparticle on gold surface for electrochemical DNA sensing and gene delivery Influence of alkane chain lengths and head groups on tethering of liposome-gold nanoparticle on gold surface for electrochemical DNA sensing and gene delivery](/preview/png/7144984.png)
چکیده انگلیسی
1,2-Dioleoyltrimethylammoniumpropane (DOTAP) liposome-gold nanoparticle (DOTAP-AuNP) is tethered on different thiol monolayers with different chain lengths (carbon number n = 3 and 10) and head groups (acid and amine) on gold transducer for applications of DNA sensing, gene transfer and antibacterial activities. Gram âve (Escherichia coli (E. coli)) and +ve (Staphylococcus aureus (S. aureus)) bacterial cells are used as model systems. The structure of planar and/or spherical liposome-AuNP is ascertained electrochemically in presence of transmembrane protein melittin and electroactive K3[Fe(CN)6]/K4[Fe(CN)6] redox couples. Results from transmission electron microscopy (TEM), ultraviolet-visible spectroscopy (UV-vis), dynamic light scattering (DLS) spectroscopic techniques and electrochemical data confirm the presence of more stable spherical structured DOTAP-AuNPs on the alkane thiols with amine head groups than on the acid terminal alkane thiols irrespective of chain length differences. Label free electrochemical DNA sensing in presence of K3[Fe(CN)6]/K4[Fe(CN)6] indicates large discrimination efficiency at concentrations greater than nano mole for the spherical DOTAP-AuNP on the aminoundecane thiol. DNA transfer into E. coli cells and anti-bacterial studies are made using gram âve and +ve E. coli cells. The DNA transfer and antibacterial activities are confirmed using differential confocal laser scanning microscopy (CLSM).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 223, February 2016, Pages 157-165
Journal: Sensors and Actuators B: Chemical - Volume 223, February 2016, Pages 157-165
نویسندگان
Mohanlal Bhuvana, Venkataraman Dharuman,