کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7145430 | 1462069 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Magnetic functionalized electrospun nanofibers for magnetically controlled ultrasensitive label-free electrochemiluminescent immune detection of aflatoxin B1
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Magnetic functionalized electrospun nanofibers for magnetically controlled ultrasensitive label-free electrochemiluminescent immune detection of aflatoxin B1 Magnetic functionalized electrospun nanofibers for magnetically controlled ultrasensitive label-free electrochemiluminescent immune detection of aflatoxin B1](/preview/png/7145430.png)
چکیده انگلیسی
A label-free electrochemiluminescent (ECL) immunoassay protocol was fabricated based on magnetic nanoarchitecture for sensitive detection of aflatoxin B1 (AFB1). It was constructed by using magnetic control technology to immobilize the magnetic nanofibers on carbon nanohorns (CNHs) matrix. First, CNHs possessed superstructure with excellent electrical conductivity, biocompatibility, large specific surface areas, and variable porosity, which could effectively amplified the ECL signal of luminol. In addition, magnetic nanofibers with magnetism could be immobilized tightly on magnetic electrode and accommodated large number of antibody on the interface. Finally, owing to the specific recognition of antibody with AFB1 antigens and the effective ECL amplification of the nanoarchitecture, the as-proposed immunosensor afforded a remarkably sensitive analysis of the AFB1 with a wide detection linear range from 0.05Â ng/mL to 200Â ng/mL and a detection limit of 0.02Â ng/mL. More importantly, the immunosensor was successfully utilized to detect practical samples. Therefore, the prepared immunosensor exhibited its reliability and feasibility. Furthermore, with the good stability, acceptable precision and reproducibility, the proposed immunosensor possessed promising practicability for other analytical fields.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 222, January 2016, Pages 707-713
Journal: Sensors and Actuators B: Chemical - Volume 222, January 2016, Pages 707-713
نویسندگان
Guifang Xu, Shupei Zhang, Qingrong Zhang, Lingshan Gong, Hong Dai, Yanyu Lin,