Electrochemical aptasensor based on one-step synthesis of Cu2O@aptamer nanospheres for sensitive thrombin detection

Cu2O@aptamer nanocomposites were synthesized using aptamer as a template by one-step reduction method and used as the sensitive layer of an immunbiosensor for thrombin detection using electrochemical techniques. The developed electrochemical biosensor based on Cu2O@aptamer nanocomposites exhibited high sensitivity for thrombin detection with a detection limit of 0.01 ng mL−1 within the range from 0.01 to 50 ng mL−1.