Enhancing sensitivity of hemoglobin-based electrochemical biosensor by using protein conformational intermediate

- Conformation of hemoglobin was accurately regulated to intermediate.
- Protein intermediate was used to construct an electrochemical sensor.
- The sensitivity of the presented senor was greatly enhanced by the intermediate.
- The method provides a new strategy to utilize the properties of redox proteins.
- The study illustrates the relationship of proteins conformation with their functions.