Planar poly(dimethylsiloxane) (PDMS)-glass hybrid microdevice for a flow-through polymerase chain reaction (PCR) employing a single heater assisted by an intermediate metal alloy layer for temperature gradient formation

The use of an Invar metal alloy as an intermediate layer between the microdevice and a heater enabled the formation of uniformly distributed temperature gradient on the planar microdevice utilizing a single heater, and various targets were successfully amplified with reliable target specificity using one microdevice.