کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7147949 | 1462100 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
DNAzyme-guided polymerization of aniline for ultrasensitive electrochemical detection of nucleic acid with bio-bar codes-initiated rolling circle amplification
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
A novel and versatile amplified electrochemical strategy for ultrasensitive detection of nucleic acid has been developed on the basis of polyaniline (PANI) as the redox label which was guided and deposited by multiple HRP-mimicking DNAzyme as a powerful catalyst and template generated by bio-bar codes-initiated rolling circle amplification (RCA). The “sandwich-type” DNA biosensor was fabricated with the thiol-functionalized capture DNA first immobilized on an Au electrode and hybridized with one end of target DNA, the other end of which was recognized with a signal DNA probe that on the bio-bar codes, respectively. After the RCA reaction, multiple HRP-mimicking DNAzyme were successfully formed, which subsequently catalyzed the oxidation of aniline to PANI and lead to a readily measurable “turn-on” electrochemical signal. As a result of multiple amplification effects, the constructed platform exhibited a good linear response toward nucleic acid over a wide range of concentration from 6.0Â fM to 1.0Â nM.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 190, January 2014, Pages 384-388
Journal: Sensors and Actuators B: Chemical - Volume 190, January 2014, Pages 384-388
نویسندگان
Ting Hou, Xiaojuan Liu, Xiuzhong Wang, Ai-wen Jiang, Shufeng Liu, Feng Li,