کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7148561 1462102 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel SPR biosensors based on metal nanoparticles decorated with graphene for immunoassay
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Novel SPR biosensors based on metal nanoparticles decorated with graphene for immunoassay
چکیده انگلیسی
Novel surface plasmon resonance (SPR) biosensors based on Au-graphene (Au-Gra) nanohybrids and Ag-graphene (Ag-Gra) nanohybrids for the detection of mouse IgG were developed. The biosensors proposed are label free, and have high sensitivity and selectivity for biosensing. Au-Gra and Ag-Gra nanohybrids were synthesized in a simple, effective and rapid way, and further characterized by X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM) and UV-vis absorption spectroscopy. These nanohybrids were assembled on the Au film through 1,6-hexanedithiol by covalent attachment. And the antibody was bound to the nanohybrids through 3-mercaptopropinic acid. Some experimental conditions were determined and optimized. In the optimal conditions, the biosensors based on Au-Gra nanohybrids and Ag-Gra nanohybrids show a good response to mouse IgG in the concentration range of 0.30-40.00 μg mL−1 and 0.15-40.00 μg mL−1, respectively. While, the biosensor unmodified with nanohybrids shows a response to mouse IgG in the concentration range of 2.50-40.00 μg mL−1. As a result, the performance of the biosensor based on metal nanoparticles decorated with graphene has been improved significantly.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 188, November 2013, Pages 548-554
نویسندگان
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