کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7148871 | 1462105 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An integrated electrophysiological and optical approach for ion channel study in a microfluidic system enabling intra- and extra-cellular solution exchange
ترجمه فارسی عنوان
یک روش الکتروفیزیولوژیک و اپتیکی یکپارچه برای مطالعه کانال یون در یک سیستم میکرو فلوئیدیک امکان تبادل محلول درونی و خارج سلولی
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The planar patch-clamp technique has revolutionized ion channel study by increasing throughput and minimizing sophisticated operation. Nevertheless, the system design is often focused toward a particular objective, which sometimes limits the range of information output. Here, we present an integrated electrophysiological and optical approach for ion channel study in a microfluidic system allowing multi-content detection. Multiple fluidic injections enable both intra- and extracellular solution exchange to facilitate the study of the ion channel in a microfluidic chip with high-yield seal formation. Whole-cell and single cell recordings were validated for various cell lines, i.e. endogenous channels of HIT-T15, CHO-K1, HEK-293T, and RIN-m5f cells. Long-term recording of temporal fluorescent changes on a trapped RIN-m5F cell shows the ability of extracellular solution exchange and simultaneous optical observation. The integrated microdevice system serves as a practical tool for high-quality and multi-content ion channel electrophysiological study.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 185, August 2013, Pages 496-503
Journal: Sensors and Actuators B: Chemical - Volume 185, August 2013, Pages 496-503
نویسندگان
Ting-Yuan Tu, Chang-Yu Chen, De-Shien Jong, Andrew M. Wo,