کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7148980 1462114 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel reagentless glucose biosensor based on ferrocene cored asymmetric PAMAM dendrimers
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Novel reagentless glucose biosensor based on ferrocene cored asymmetric PAMAM dendrimers
چکیده انگلیسی
A novel amperometric glucose biosensor is fabricated with immobilization of glucose oxidase (GOx) onto PAMAM-Fc dendrimers decorated gold electrode. Series of asymmetric PAMAM dendrimers containing a single ferrocene unit located in the focal point of these macromolecules have been synthesized and characterized. Surface of the gold electrode was covalently modified with 3-mercaptopropionic acid, PAMAM-Fc dendrimers and GOx enzyme by using coupling agents, respectively. The PAMAM-Fc/GOx biosensor shows an excellent performance for glucose at +0.25 V with a high sensitivity (6.54 μA/mM) and lower response time (∼3 s) in a wide concentration range of 1-22 mM (correlation coefficient of 0.9988). In addition, PAMAM-Fc/GOx biosensor possesses better reproducibility, storage stability and there is negligible interference from other electroactive components.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 176, January 2013, Pages 299-306
نویسندگان
, , ,