کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7207406 1469068 2018 38 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Multi-tubule conduit-filler constructs loaded with gradient-distributed growth factors for neural tissue engineering applications
ترجمه فارسی عنوان
ساختارهای کانال پرکننده چند لوله ای که با استفاده از فاکتور رشد توزیع شده شیب برای برنامه های کاربردی مهندسی بافت پر شده است
کلمات کلیدی
ساختار پرکننده لوله چند لوله ای، تنزل، خاصیت مکانیکی، فاکتور رشد عصبی، توزیع گرادیان،
موضوعات مرتبط
مهندسی و علوم پایه سایر رشته های مهندسی مهندسی پزشکی
چکیده انگلیسی
Chitosan/silk fibroin/glycerophosphate gels were loaded with nerve growth factor (NGF) and further processed into multi-tubule fillers. NGF was loaded into the fillers in such a way so that a NGF gradient was established longitudinally along the filler length. A type of poly(lactide-co-glycolide)(PLGA)/chitosan(CH) porous conduit was fabricated using a pre-crosslinking method. The filler was fully filled into the lumen of conduits to build multi-tubule conduit-filler constructs that are intended for long-gap peripheral nerve repair. In vitro degradation in a lysozyme-contained medium revealed that constructs had degradation-tolerant features and the optimized multi-tubule filler was capable of maintaining its multi-tubules unblocked for around 10-week. After being degraded for various periods up to 8 weeks, the optimal conduit-filler constructs showed confirmative ability to retain their compressive load, deformation recovery and tensile strength at about 80 N/m, 75% and 15 N/cm2 in wet state, respectively. The constructs were able to administer NGF release and to maintain persistent NGF gradients longitudinally distributed inside the PLGA/CH conduit for about 6 weeks or even longer. The PC12 cell neurite extension assay confirmed that the presently developed multi-tubule conduit-filler constructs were reliable for effectively preserving the bioactivity of released NGF.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the Mechanical Behavior of Biomedical Materials - Volume 77, January 2018, Pages 671-682
نویسندگان
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