BIOMASS GROWTH AND kLa ESTIMATION USING ONLINE AND OFFLINE MEASUREMENTS

Measurement of the key process variables is essential during biopharmaceutical production. These measurements are often not available online. This work combines frequent online measurements with infrequent offline measurements to estimate the specific growth rate, biomass, and the oxygen mass transfer coefficient during continuous and fed-batch cultivations of Bordetella pertussis online using an Extended Kalman filter, parameter adaptation, and learning.