MODEL FORMULATION FOR HYBRIDOMA CULTURES IN BATCH AND FED-BATCH MODE

Metabolic Flux Analysis was performed to identify the significant metabolic reactions for hybridoma cells during batch and fed-batch culture. Correlation analysis yielded the factors that influence biomass growth and productivity and elucidated the nature of the relationship between them. Consequently, an integrated dynamic model expressing the rate of consumption and production of nutrients/metabolites and the biomass growth and decline was developed.