کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
724098 | 892361 | 2007 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
DEVELOPMENT OF A NEW PROBE FOR IN-SITU OXYGEN UPTAKE RATE (OUR) MEASUREMENT IN MAMMALIAN CELL CULTURE PROCESSES
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
سایر رشته های مهندسی
مکانیک محاسباتی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have developed a new probe that measures the oxygen uptake rate (OUR) of mammalian cells, an important indicator for the metabolic state, inside the reactor (in-situ) and in real-time without the need for sampling or complicated analytics. The probe isolates a known volume of cell culture from the bulk inside the bioreactor, monitors the oxygen consumption over time and releases it again. The sample is mixed during the measurement with a new agitation system to keep the cells in suspension and prevent oxygen concentration gradients. The OUR measurement system also doubles as a standard DO probe for process monitoring when it is not performing OUR measurements. This new probe was successfully tested in BHK perfusion cell cultures.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: IFAC Proceedings Volumes - Volume 40, Issue 4, 2007, Pages 273–276
Journal: IFAC Proceedings Volumes - Volume 40, Issue 4, 2007, Pages 273–276
نویسندگان
K. Joeris, L. Behr, M. Burnett, T. Scheper, K. Konstantinov,