The effect of calibration equations on the uncertainty of UV–Vis spectrophotometric measurements

The effect of adequateness of the calibration equations on measurement uncertainty was not mentioned by EURACHEM. In this work, we investigate the sources of uncertainty when measuring glucose concentration with a UV–Vis spectrophotometer. The effect of two calibration equations on the uncertainty was compared. The sources of the glucose concentration measurement uncertainty include purity, volume of flasks, mass and the calibration equations. The effects of two calibration equations, linear and polynomial equation, on the uncertainty source were evaluated using the inverse calibration equation. The results indicated that the uncertainty components from purity were the smallest. The volumes of the volumetric flasks had only modest effect on the uncertainty, while the mass was an important source of the uncertainty. The contribution of the calibration equation to the total relative measurement uncertainty was 59.39% for the linear equation and 30.34% for the polynomial equation. With the selection of the adequate equation, the uncertainty source of the calibration equation could be reduced significantly.