کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
740104 | 1462097 | 2014 | 7 صفحه PDF | دانلود رایگان |
• A biosensor for the determination of Cd2+ based on horseradish peroxidase enzymatic inhibition has been constructed.
• The properties of the constructed biosensor was studied by UV–Vis and cyclic voltammetry.
• An inhibition constant, Ki (0.018 mg L−1) was determined by using the Dixon plot.
• The type of inhibition was found to be non-competitive using Dixon and Cornish-Bowden plots.
• The biosensor was suitable for Cd2+ detection in natural water standard reference material®.
The present work is focused on the application of a hydrogen peroxide (H2O2) biosensor for the detection of heavy metals ions. The biosensor was constructed by physical adsorption of horseradish peroxidase (HRP) onto a maize tassel (MT)-multiwalled carbon nanotube (MWCNT) composite and was characterized using spectroscopic and voltammetric methods. UV–Vis results inferred that HRP was not denatured during its immobilization on MT–MWCNT composite. The biosensing principle was based on the determination of the cathodic responses of the immobilized HRP to H2O2, before and after incubation in trace metal standard solutions. Using Cd2+ as a model metal ion, the inhibition rate of the trace metal was proportional to concentration in the range of 2–30 μg L−1 with a limit of detection of 0.51 μg L−1. Representative Dixon and Cornish-Bowden plots showed that the reaction was non-competitive. The developed biosensor exhibited good stability, repeatability and reproducibility, thus providing a new promising tool for analysis of enzyme inhibitors.
The modification and inhibition processFigure optionsDownload as PowerPoint slide
Journal: Sensors and Actuators B: Chemical - Volume 193, 31 March 2014, Pages 515–521