کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
740305 | 1462104 | 2013 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Incident-angle dependence of fluorescence enhancement and biomarker immunoassay on gold nanohole array Incident-angle dependence of fluorescence enhancement and biomarker immunoassay on gold nanohole array](/preview/png/740305.png)
We theoretically and experimentally investigate the influence of the incident angle of illumination on the fluorescence enhancement for two kinds of gold nanohole arrays – a gold nanohole array on glass substrate (Au-hole array), and a gold coated photoresist nanohole array (Au/PR-hole array). Our simulations show that the absorption spectra for the Au/PR-hole array vary substantially with angle of incidence, whereas the corresponding spectra for the Au-hole array are marginally affected. The experimental results reveal fluorescence enhancement up to 14 and 12 times for the Au-hole array at an incident angle of 70° and the Au/PR-hole array at an incident angle of 20°, respectively. Moreover, a model sandwich immunoassay for the detection of prostate specific antigen (PSA) shows about 3-fold higher sensitivity on Au/PR-hole array as compared to planar gold at 70°. To the best of our knowledge this is the first investigation reporting on the relationship between the incident angle and the fluorescence enhancement on gold nanohole arrays. Our findings provide a solid foundation for designing a highly efficient fluorescence detection system with oblique angular illumination.
Journal: Sensors and Actuators B: Chemical - Volume 186, September 2013, Pages 205–211