کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
740743 | 894186 | 2011 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Conducting polymers with benzothiadiazole and benzoselenadiazole units for biosensor applications Conducting polymers with benzothiadiazole and benzoselenadiazole units for biosensor applications](/preview/png/740743.png)
Poly(4,7-di(2,3)-dihydrothienol[3,4-b][1,4]dioxin-5-yl-benzo[1,2,5]thiadiazole) (PBDT) and poly(4,7-di(2,3)-dihydrothienol[3,4-b][1,4]dioxin-5-yl-2,1,3-benzoselenadiazole) (PESeE) were electrochemically deposited on graphite electrodes and used as immobilization matrices for biosensing studies. After electrochemical deposition of the polymeric matrices, glucose oxidase (GOx) was immobilized on the modified electrodes as the model enzyme. In the biosensing studies, the decrease in oxygen level as a result of enzymatic reaction was monitored at −0.7 V vs Ag/AgCl (3.0 M KCl) and correlated with substrate concentration. The biosensor was characterized in terms of several parameters such as operational and storage stabilities, kinetic parameters (Km and Imax) and surface morphologies. The biosensor was tested on real human blood serum samples.
► A durable assembly of enzyme and a polymer even with no covalent binding.
► Analysis of glucose content in human blood samples.
► Substantial shelf-life activity.
Journal: Sensors and Actuators B: Chemical - Volume 158, Issue 1, 15 November 2011, Pages 117–123