کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
742724 1462088 2014 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
ELISA type fluorescence turn-on immunoassay based on Fe3+ induced fluorescence enhancement
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
ELISA type fluorescence turn-on immunoassay based on Fe3+ induced fluorescence enhancement
چکیده انگلیسی

ELISA type fluorescence immunoassay for the sensitive detection of protein biomarker tumor necrosis factor α (TNF-α) was proposed. The immuno-reaction was taken place in the wells of the 96 well plate using Fe3O4 nanoparticles as label. The signal amplification strategy was based on the released Fe3+ from Fe3O4 nanoparticles after acid treatment to trigger the fluorescence from nonfluorescent fluorophore. The fluorophore selected was rhodamine 6G derivative N-(rhodamine-6G)lactam-ethylenediamine (Rh6G-LEDA). Such signal turn-on strategy has advantages of low background current and less susceptibility to fluorescence quench. The immunoassay displays high sensitivity, wide linear range and good selectivity for TNF-α detection. The developed immunoassay combines the high throughput of ELISA type assay and the low cost as well as simplicity of nanoparticle label, which could find wide applications for clinical screening purposes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 202, 31 October 2014, Pages 663–666
نویسندگان
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