کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
742968 894337 2010 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Numerical modeling of the effect of histidine protonation on pH distribution and DNA hybridization in electronically active microarrays
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Numerical modeling of the effect of histidine protonation on pH distribution and DNA hybridization in electronically active microarrays
چکیده انگلیسی

This study investigates, through numerical modeling, the effects of protonation of histidine buffer in promoting DNA hybridization in electronically active microarrays within a limited pH range. The numerical model framework developed in this study consists of a number of physical phenomena (conservation and mass transport of species) and chemical equilibrium reactions (hydrolysis of water, heterogeneous DNA hybridization, and protonation of histidine) that govern the hybridization of single-stranded DNA molecules (ssDNA) in active microarrays within an environment of continuous generation of H+ ions and their subsequent consumption by histidine buffer. The study demonstrates, through a numerical model and comparison with published experimental results, that the increase in acidity near anodes due to the hydrolysis of water can be neutralized by a histidine buffer creating a relatively stable pH environment. To account for the dependency of DNA hybridization on the pH of the buffer used, the study also introduces a new hybridization efficiency parameter into the numerical model that is based on experimental data for DNA hybridization.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 143, Issue 2, 7 January 2010, Pages 470–481
نویسندگان
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