A high selective disposable biosensor based on screen-printed technique with two working electrodes for eliminating interference signals

A novel disposable biosensor containing a detection electrode and a control electrode was constructed by the screen-printing techniques. The detection electrode was covered with the enzyme ink to measure the oxidation currents of the catalytic substrate, while the control electrode was coated with non-enzyme ink to monitor the interference signals. The net catalytic currents of the enzyme electrode can be obtained from the response differences of the two recording electrodes. The biosensor for detection of glucose in blood sample showed a good linear relationship in the range of 0.5–50 mM (R = 0.9986) with a detection limit of 0.05 mM (S/N = 3), and displayed a high selectivity. The general applicability of the biosensor was demonstrated by successfully using it for detecting uric acid in blood sample. The proposed biosensor possesses a high selectivity and accuracy for detecting species in biological samples, which opens the possibility of developing screen-printed electrode-based devices for bioanalysis.