کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
743335 1462116 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Multi-resistance as a tool for detecting novel beta-lactam antibiotics in the environment
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Multi-resistance as a tool for detecting novel beta-lactam antibiotics in the environment
چکیده انگلیسی

Over the past two decades, antibiotic resistance has become a major clinical and public health problem. The requirement that conventional antibiotics be used at high concentrations and the inability to determine specific antibacterial modes of action against the target cells make conventional methods (e.g., screening large libraries of natural or synthetic molecules) less efficient. This study demonstrates a novel method for the detection of new antibacterial compounds using genetically modified bacteria. Different multi-resistant (MR) bioluminescent strains were developed and exposed to the various β-lactam antibiotics. We show that this MR strain panel is able to determine a specific antibiotic to which a bacterium is not resistant. Furthermore, the MR panel allows the detection of biologically active compounds derived from the environment, and in some cases, these compounds inhibited all bioreporters used, showing that no bacteria were resistant to them. This MR strain panel offers a unique opportunity to improve the biodiscovery of new antibiotics with unknown modes of action, a finding that may facilitate the development of new and effective drugs to which existing bacteria are not resistant

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 174, November 2012, Pages 342–348
نویسندگان
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