کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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743948 | 1462123 | 2011 | 6 صفحه PDF | دانلود رایگان |
An in situ method for modifying a receptor site on mesoporous silica MCM-41 channels in planar lipid bilayers is described, in which bovine serum albumin (BSA) is covalently linked to the MCM-41 channels via head groups of lipids loaded in the nanopores. Prior to receptor modification, lipid-loaded MCM-41 channels were incorporated with lipid bilayers formed at an aperture of a Teflon film. The in situ coupling of BSA to lipid-loaded MCM-41 channels at the lipid bilayer interface was achieved by the sulfhydryl coupling method. The lipid bilayers containing BSA-modified MCM-41 exhibited channel-like currents, which were augmented in a concentration-dependent manner by the addition of anti-BSA at fM level. The in situ modification of lipid-loaded MCM-41 channels with BSA by the amine coupling technique was also investigated. The potential of the present approach for the development of channel-type biosensors is discussed in terms of modifying bilayer interfaces with bioreceptors.
Journal: Sensors and Actuators B: Chemical - Volume 160, Issue 1, 15 December 2011, Pages 139–144