کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
744109 | 894381 | 2011 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A disposable amperometric biosensor for determining total cholesterol in whole blood A disposable amperometric biosensor for determining total cholesterol in whole blood](/preview/png/744109.png)
A disposable amperometric biosensor for detecting the total cholesterol was fabricated which comprises a sensing electrode and a reference electrode in simultaneous contact with an integrated reagent layer. The integrated reagent layer formed by coating a working ink containing cholesterol esterase, cholesterol dehydrogenase, coenzyme, redox mediator, surfactant, stabilizer, filler and at least one aqueous thickening agent. The biosensor showed the linearity for 50–500 mg/dL cholesterol acetate. The minimum detection limit of the cholesterol was 50 mg/dL. The effects of temperature and the stability of immobilized enzymes were also studied. A good correlation was found among cholesterol values obtained by commercial colorimetric test strip and clinical/laboratory methods. The biosensor showed an acceptable reproducibility, good stability and low interferences.
Journal: Sensors and Actuators B: Chemical - Volume 155, Issue 2, 20 July 2011, Pages 545–550