کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
744239 | 1644971 | 2016 | 7 صفحه PDF | دانلود رایگان |
• PyCM showed a remarkable fluorescence “turn-on” response to Au3+/Au+ in aqueous solution.
• The Au3+-induced CN bond hydrolysis sensing mechanism was fully confirmed.
• PyCM can be used to detect Au3+ in living cells under two-photon excitation.
• PyCM can be predominantly present in mitochondria (co-localization coefficient: 0.85).
A carbazole-based two-photon fluorescent probe (PyCM) showed a remarkable fluorescence “turn-on” response to Au3+/Au+ in aqueous solution with a large Stokes shift (119 nm). A good linear relationship of emission intensity at 539 nm against Au3+/Au+ from 0 to 20 equiv. was observed, and the detection limits (3σ/k) were as low as 47 nM for Au3+ and 73 nM for Au+, respectively. The fluorescence enhancement was attributed to the gold ions-induced CN bond hydrolysis sensing mechanism, which is fully confirmed by the UV–vis absorption, fluorescence, IR, 1H NMR titration and MALDI-TOF mass analysis. Bio-imaging study established that PyCM could be used to detect Au3+ in living cells under two-photon excitation with large two-photon absorption cross sections (1321 GM at 860 nm), little cytotoxicity and good biocompatibility. Meanwhile, standard co-staining experiments of PyCM and MitoTracker Red FM (co-localization coefficient: 0.85) revealed that PyCM was predominantly present in mitochondria.
A mitochondria-targeted two-photon fluorescent probe (PyCM) was developed for highly selective detection of gold ions and bio-imaging in mitochondria under two-photon excitation.Figure optionsDownload as PowerPoint slide
Journal: Sensors and Actuators B: Chemical - Volume 225, 31 March 2016, Pages 572–578