Ag/Au nanoparticles coated graphene electrochemical sensor for ultrasensitive analysis of carcinoembryonic antigen in clinical immunoassay

• A new electrochemical sensor for determination of carcinoembryonic antigen was developed.
• The Ag/Au nanoparticles coated on graphene was used as amplifier of signals.
• The ultrasensitive method could be successfully applied for biosample analysis.

This study demonstrates a novel approach toward development of advanced immunosensors based on chemically functionalized Ag/Au nanoparticles coated on graphene for the detection of carcinoembryonic antigen (CEA) in clinical immunoassay. Ag/Au nanoparticles were synthesized and then were used as a signal amplifying factor for construction of the “sandwich-type” immunosensor. The Ag/Au nanostructure presents good electrochemical activity in the working potential range, which provides high current response. Under optimal conditions, the proposed method could respond to carcinoembryonic antigen (CEA) with a linear calibration range from 10 to 1.2 × 105 pg mL−1 with a detection limit of 8 pg mL−1. This new biosensor exhibited high sensitivity and selectivity, and was successfully used in determining the CEA which was added in human serum.

A novel approach toward development of advanced immunosensors based on chemically functionalized Ag/Au nanoparticles coated on graphene was developed for the detection of carcinoembryonic antigen (CEA) in clinical immunoassay.Figure optionsDownload as PowerPoint slide