A colorimetric biosensor for the detection of foodborne bacteria

We have synthesized 10,12-pentacosadyinoic acid (PCDA) + N-[(2-tetradecanamide)-ethyl]-ribonamide (TDER) vesicles to determine the colorimetric response induced by pathogenic bacteria (Staphylococcus aureus and Escherichia coli). The addition of bacterial supernatants caused a colorimetric transition in TDER/PCDA vesicles, even in diluted concentrations, indicating that chemical interactions occur between the vesicles and bacterial compounds. Bacterial substrates released from S. aureus induced a greater color change compared to the color change induced by E. coli. S. aureus metabolites also induced a greater color change when TDER/PCDA vesicles were incorporated into the cellulose strips. We determined the energy involved in the interaction between the bacterial substrates and the TDER/PCDA vesicles to be exothermic (and very high) for both bacterial supernatants. In addition, we analyzed the colorimetric transition in the presence of other molecules, using apple juice as a food matrix. Both apple juice and apple juice inoculated with bacterial substrates induced a TDER/PCDA color change; however, the S. aureus supernatant induced a slightly greater color change in the vesicles both in the suspension and in the cellulose strips. TDER/PCDA vesicles show great potential to be used as a biosensor to detect food pathogens in intelligent food packaging.