کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
747262 894515 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Direct electrochemistry of horseradish peroxidase immobilized on gold colloid/cysteine/nafion-modified platinum disk electrode
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Direct electrochemistry of horseradish peroxidase immobilized on gold colloid/cysteine/nafion-modified platinum disk electrode
چکیده انگلیسی

Direct electron transfer process of immobilized horseradish peroxidase (HRP) on a gold colloid-cysteine-nafion membrane, and its application as a biosensor were investigated by using electrochemical methods. The electrochemical characteristics of the biosensor were studied by cyclic voltammetry, linear sweep voltammetry and chronoamperometry. The modified process was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The sensor displayed an excellent electrocatalytic response to the reduction of H2O2 without the aid of an electron transfer mediator. Analytical parameters such as pH and temperature were also studied. Linear calibration for H2O2 was obtained in the range 3.50 × 10−7 to 5.87 × 10−3 M under the optimized conditions. The sensor was highly sensitive to H2O2 with a detection limit of 1.05 × 10−7 M (S/N = 3), and the sensor achieved 95% of the steady-state current within 10 s. The sensor exhibited high sensitivity, selectivity and stability.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 115, Issue 1, 23 May 2006, Pages 109–115
نویسندگان
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