کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
750910 | 1462094 | 2014 | 8 صفحه PDF | دانلود رایگان |
In this study we show, for the first time, that gold nanoparticles (AuNPs) synthesized by a simple, inexpensive, and environmentally-correct method can be easily conjugated with the antibodies anti-β-catenin and anti-E-cadherin to specifically target colorectal carcinoma cells. The antibody/AuNPs conjugates were then successfully applied for imaging cancerous cells with fluorescence confocal microscopy. The AuNPs as well as the conjugates were very stable in high-salinity medium, a pre-requisite for application in physiological-like environments. Fluorescence results suggest that conjugation was achieved by direct adsorption of antibodies on the AuNPs surface. Finally, compared with a standard method of cell staining, our method is less laborious and the preparation time (from immobilization of cells onto glass cover slips until observation by confocal microscopy) decreased from 27 h to about 1 h, which makes the method eligible for colorectal cancer diagnostic.
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Journal: Sensors and Actuators B: Chemical - Volume 196, June 2014, Pages 306–313