کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
751067 895188 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of sulfhydrylated antibody functionalized microcantilever immunosensor for taxol
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development of sulfhydrylated antibody functionalized microcantilever immunosensor for taxol
چکیده انگلیسی

An immobilization method using a sulfhydrylated monoclonal antibody (mAb) on the gold surface of a microcantilever immunosensor was developed for label-free detection of taxol. The sulfhydrylated anti-taxol mAb was synthesized. Then it was immobilized on the gold surface of the microcantilever. The deflection of the microcantilever corresponding to different taxol concentrations was real time monitored by optical lever technique. The activity of the anti-taxol mAb before and after sulfhydrylation, and the binding of the sulfhydrylated anti-taxol mAb on the gold surface of the microcantilever were evaluated by non-competitive enzyme-linked immunosorbent assay (ELISA). The limit of detection is 1 ng/mL and the method was used for detection of taxol in human plasma sample.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 156, Issue 2, August 2011, Pages 863–866
نویسندگان
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