کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
751190 895206 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel immunosensor platform based on inorganic barium strontium titanate film for human IgG detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Novel immunosensor platform based on inorganic barium strontium titanate film for human IgG detection
چکیده انگلیسی

In this investigation, we report a novel immunosensor platform using sol–gel derived amorphous barium strontium titanate (BST) thin films. The impedimetric immunosensor detection was carried out based on a redox-free system with two electrodes and a very diluted NaCl solution as electrolyte. As a proof of concept, the anti-human IgG, a common biomarker found and associated with many diseases, was firstly immobilized on the immunosensor surface, pre-modified by 3-aminopropyltriethoxysilane (APTS) and then by the coupling agent of glutaraldehyde. The modified sensor surface served as selective sensing probe to capture/conjugate the human IgG, pre-diluted with phosphate buffered saline (PBS). Large detectable impedance shift at the low frequency range was observed in an up-trend on antigen immobilization and antigen–antibody conjugation. The measured signal changes in impedance were attributed mainly to the dipole induced interfacial polarization potential at the film/biomolecules interface. Good and repeatable linear relationship was found between the electrical signal outputs and human IgG concentration in logarithm scale for a wide range of antibody concentrations. A detection limit of around 40 ng/mL was also observed. This BST-based immunosensor platform has proven to be effective and promising for biological detection.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 149, Issue 2, 19 August 2010, Pages 381–388
نویسندگان
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