Direct electrochemistry of hemoglobin immobilized on siliceous mesostructured cellular foam

The direct electrochemistry of hemoglobin (Hb) immobilized on the siliceous mesostructured cellular foam (MCF) modified glassy carbon electrode (GC) was presented. Using a simple physical adsorption method, Hb was immobilized on MCF and the influences of concentration of Hb and the pH value of solution were investigated. UV-vis and FTIR spectra displayed that Hb maintained its natural structure inside the pores of MCF. The electrochemical property of Hb/MCF/GC modified electrode was studied by cyclic voltammetry. A couple of quasi-reversible redox peaks of Hb were observed on the Hb/MCF/GC electrode at about −0.396 V and −0.321 V in PBS (pH 7.0). The results indicated that MCF provided a favorable microenvironment for immobilization of Hb and facilitated the electron transfer between Hb and electrode surface.