کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
751793 | 895261 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
FTIR-ATR detection of proteins and small molecules through DNA conjugation
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
With the help of chemical modification of silicon surfaces, FTIR-ATR was developed into a biosensing method to monitor biochemical processes on surfaces. In this proof-of principle work, FTIR-ATR crystals were covalently modified by oligonucleotides and two model systems were investigated, including recognition of anti-thrombin aptamer with thrombin and organic molecule binding to double helix DNA with sequence-specific photocleavage. The affinity of aptamer recognition and the affinity of photocleavage reagent towards double helix of DNA were quantitatively measured. Thus, it was shown that the proposed FTIR-ATR-based biosensing method allows for high-sensitive label-free detection of various biological systems.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 114, Issue 1, 30 March 2006, Pages 445–450
Journal: Sensors and Actuators B: Chemical - Volume 114, Issue 1, 30 March 2006, Pages 445–450
نویسندگان
Wei Liao, Fang Wei, Dan Liu, Min Xie Qian, Gu Yuan, Xin Sheng Zhao,