کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7556655 1491293 2018 33 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel approach for amine derivatization of MoS2 nanosheets and their application toward label-free immunosensor
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A novel approach for amine derivatization of MoS2 nanosheets and their application toward label-free immunosensor
چکیده انگلیسی
The application of molybdenum disulfide (MoS2) nanosheets has assumed great significance in the design of next-generation biosensors. The immobilization of biomolecules on MoS2 nanosheets has generally been achieved via hydrophobic interactions or through other complicated surface modifications. In this work, we report a novel strategy for electrochemically assisted amine derivatization of MoS2 nanosheets. This newly proposed approach helped to immobilize the MoS2 nanosheets with antibodies via facile EDC/NHS {N-(3-dimethylaminopropyl)-N-(ethylcarbodiimide/N-hydroxysuccinimide)} cross-linking chemistry. The MoS2 nanosheets were first exfoliated and then electrochemically modified with 2-aminobenzylamine. Through a subsequent bioconjugation of the above amine-derivatized MoS2 nanosheets with anti-prostate-specific antigen (PSA) antibodies, an immunosensing device was realized for the detection of the 'prostate specific antigen'. The application of the proposed immunosensor was characterized with a low detection limit (10−3 ng/mL) over a very wide quantitation range (10−3 to 200 ng/mL).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 555, 15 August 2018, Pages 1-8
نویسندگان
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