کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7556876 | 1491299 | 2018 | 20 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Selection of specific aptamer against enrofloxacin and fabrication of graphene oxide based label-free fluorescent assay
ترجمه فارسی عنوان
انتخاب آپتامر اختصاصی در برابر انروفلوکساسین و ساخت اکسید گرافن مبتنی بر تست فلورسنت رایگان
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Specific ssDNA aptamers for the antibiotic enrofloxacin (ENR) were isolated from an enriched nucleotide library by SELEX (Systematic Evolution of Ligands by EXponential enrichment) method with high binding affinity. After seven rounds, five aptamers were selected and identified. Apt58 with highest affinity and sensitivity (Kdâ¯=â¯14.19â¯nM) was employed to develop a label-free fluorescent biosensing approach based on aptamer, graphene oxide (GO) and native fluorescence of ENR for determination of ENR residue in raw milk samples. Under optimized experimental conditions, the linear range was from 5â¯nM to 250â¯nM and LOD was calculated to be 3.7â¯nM, and the recovery rate was between 94.1% and 108.5%. The integration of aptamer and GO in this bioassay provides a promising way for rapid, sensitive and cost-effective detection of ENR in real samples like raw milk.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 549, 15 May 2018, Pages 124-129
Journal: Analytical Biochemistry - Volume 549, 15 May 2018, Pages 124-129
نویسندگان
Somayeh Dolati, Mohammad Ramezani, Maryam Sadat Nabavinia, Vahid Soheili, Khalil Abnous, Seyed Mohammad Taghdisi,