کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7558118 1491363 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Magnetic beads-based electrochemical immunosensor for monitoring allergenic food proteins
ترجمه فارسی عنوان
ایمونسنسور الکتروشیمیایی بر پایه مغناطیسی برای نظارت بر پروتئین های غذای آلرژیک
کلمات کلیدی
ایمونوسانسور الکتروشیمیایی، ذرات مغناطیسی، اوالوبومین،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Screen-printed platinum electrodes as transducer and magnetic beads as solid phase were combined to develop a particle-based electrochemical immunosensor for monitoring the serious food allergen ovalbumin. The standard arrangement of enzyme-linked immunosorbent assay became the basis for designing the immunosensor. A sandwich-type immunocomplex was formed between magnetic particles functionalized with specific anti-ovalbumin immunoglobulin G and captured ovalbumin molecules, and secondary anti-ovalbumin antibodies conjugated with the enzyme horseradish peroxidase were subsequently added as label tag. The electrochemical signal proportional to the enzymatic reaction of horseradish peroxidase during the reduction of hydrogen peroxide with thionine as electron mediator was measured by linear sweep voltammetry. The newly established method of ovalbumin detection exhibits high sensitivity suitable for quantification in the range of 11 to 222 nM and a detection limit of 5 nM. Magnetic beads-based assay format using external magnets for rapid and simple separation has been proven to be an excellent basis for electrochemical detection and quantification of food allergens in highly complex sample matrices.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 484, 1 September 2015, Pages 4-8
نویسندگان
, , , , , , ,