کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7559146 1491386 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
NqA: An R-based algorithm for the normalization and analysis of microRNA quantitative real-time polymerase chain reaction data
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
NqA: An R-based algorithm for the normalization and analysis of microRNA quantitative real-time polymerase chain reaction data
چکیده انگلیسی
In this note, we propose an R function named NqA (Normalization qPCR Array, where qPCR is quantitative real-time polymerase chain reaction) suitable for the identification of a set of microRNAs (miRNAs) to be used for data normalization in view of subsequent validation studies with qPCR data. NqA is available through the website of the Fondazione IRCCS Istituto Nazionale dei Tumori of Milan (http://www.istitutotumori.mi.it/modules.php?name=Content&pa=showpage&pid=812) with a dedicated user's guide. We applied our function on a qPCR dataset downloaded from the Gene Expression Omnibus (GEO) database. Results show that NqA provides a functional subset of reference miRNAs and a set of promising significantly modulated miRNAs for subsequent validation studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 461, 15 September 2014, Pages 7-9
نویسندگان
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