کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7559818 | 1491408 | 2010 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Exploring minimal biotinylation conditions for biosensor analysis using capture chips
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Using Biacore's new regenerateable streptavidin capture (CAP) sensor chips, we investigated a number of biotinylation conditions for target ligands. We explored standard amine as well as the less commonly used carboxyl biotinylation methods. We illustrate the time scales required for efficient biotinylation as well as the hazards of overbiotinylation. We evaluated a range of desalting methods, including spin columns, dialysis membranes, and filters. Finally, we tested the effects of common buffer components, such as Tris and glycerol, on the biotinylation process. Together, our results serve as a general guide of the steps to consider when minimally biotinylating a target ligand.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 403, Issues 1â2, August 2010, Pages 30-35
Journal: Analytical Biochemistry - Volume 403, Issues 1â2, August 2010, Pages 30-35
نویسندگان
Giuseppe Papalia, David Myszka,