Effect of basic residue on the kinetics of peptide fragmentation examined using surface-induced dissociation combined with resonant ejection

- Fragmentation kinetics of KYGGFL and RYGGFL examined using resonant ejection SID.
- RRKM modeling provides dissociation parameters for individual reaction channels.
- Basic residue has a pronounced effect on the kinetics of the b2 ion formation Similar kinetics of the b5+H2O ion formation observed for KYGGFL and RYGGFL .
- Strikingly different kinetics of the b2 ion formation is attributed to different mechanisms
- A salt-bridge pathway (RYGGFL) and canonical pathway (KYGGFL) of the b2 ion formation.