Analysis of nucleosides using the atmospheric-pressure solids analysis probe for ionization

► ASAP is an ionization technique suitable for non-polar compounds. ► Nucleosides are considered to be non-volatile compounds. ► This work shows that nucleosides are ionizable with ASAP. ► The method works also for naturally occurring and synthetically modified nucleosides. ► Presence of cations is not as critical as with electrospray ionization.