Hydrogen/deuterium exchange mass spectrometry for characterizing phosphoenolpyruvate-induced structural transitions in Mycobacterium tuberculosis 5-enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.1)

H/D exchange monitored by ESI-MS and MS/MS was used to investigate PEP-induced conformational changes in MtEPSPS.