Sugaring-out assisted liquid/liquid extraction with acetonitrile for bioanalysis using liquid chromatography-mass spectrometry

► Sugaring-out assisted liquid/liquid extraction (SULLE) has been successfully used to extract HIV drugs from human plasma. ► The assay has been validated for regulated uses and ~ 10 other compounds have been tested with SULLE with success. ► This article would be one of the earliest applications of SULLE in analytical chemistry. ► SULLE is new and more application and theory articles are anticipated in next a few years.