Metals in the water, sediment, and tissues of two fish species from different trophic levels in a subtropical Brazilian river

► We analyzed two sampling sites with different pollution degree in the same River. ► For both sites, metal concentration was highest in the sediment, middle in water, and lower in the fish liver. ► The detritivore species presented the highest concentration for all analyzed metals. ► Potential ecological risk for single regulator indexes showed low potential ecological risk for all analyzed metals except Cd.