Quantification of T-2 and HT-2 mycotoxins in cereals by liquid chromatography-multimode ionization-tandem mass spectrometry

► A new LC-MS/MS method is developed and validated for T-2 and HT-2 mycotoxins. ► Different ion modes should be applied for the toxins in the multimode ion source. ► Strata-XL SPE cartridge results in the best recovery for both toxins in flour. ► The method was applied to cereal proficiency test samples with success.