کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7704444 | 1496891 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Electrochemical immunosensor for highly sensitive and quantitative detection of tumor necrosis factor-α in human serum
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
الکتروشیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Highly sensitive and selective biosensors for accurate determination of specific protein biomarkers at low levels in serum are a prerequisite for the present healthcare systems. Therefore, here we developed a label-free impedimetric method for tumor necrosis factor-alpha (TNF-α) detection using reduced graphene oxide (RGO) with gold nanoparticles (AuNP) on an indium tin oxide (ITO) microdisk electrodes. The detection mechanism relies on resistance change occurs due to [Fe(CN)6]3â/4â redox probe movement towards the conductive channels of the AuNP-RGO films gated by the recognition of the target biomarker by its anti-TNF-α antibody. The conductivity of the AuNP-RGO structures enhanced by 10-fold in comparison with bare electrode chips. The observed resistance changes at 2 Hz (ÎR at 2Hz) enabled the quantification of various concentrations of TNF-α in human serum (CHS-TNF-α). The antibody-adsorbed electrode showed a good increment of resistance change (ÎR) with additions of antigen concentration. The sensor possesses a linear range of 1-1000 pg/ml had a detection limit of 0.67 pg/ml (38.51 fM) and 0.78 pg/ml (44.83 fM) in PBS and human serum, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 122, August 2018, Pages 93-102
Journal: Bioelectrochemistry - Volume 122, August 2018, Pages 93-102
نویسندگان
Ajay Kumar Yagati, Min-Ho Lee, Junhong Min,