کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7704636 1496892 2018 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array
موضوعات مرتبط
مهندسی و علوم پایه شیمی الکتروشیمی
پیش نمایش صفحه اول مقاله
Characterization and electrochemical response of DNA functionalized 2 nm gold nanoparticles confined in a nanochannel array
چکیده انگلیسی
Polyvalent gold nanoparticle oligonucleotide conjugates are subject of intense research. Even though 2 nm diameter AuNPs have been previously modified with DNA, little is known about their structure and electrochemical behavior. In this work, we examine the influence of different surface modification strategies on the interplay between the meso-organization and the molecular recognition properties of a 27-mer DNA strand. This DNA strand is functionalized with different sulfur-containing moieties and immobilized on 2 nm gold nanoparticles confined on a nanoporous alumina, working the whole system as an electrode array. Surface coverages were determined by EXAFS and the performance as recognition elements for impedance-based sensors is evaluated. Our results prove that low DNA coverages on the confined nanoparticles prompt to a more sensitive response, showing the relevance in avoiding the DNA strand overcrowding. The system was able to determine a concentration as low as 100 pM of the complementary strand, thus introducing the foundations for the construction of label-free genosensors at the nanometer scale.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 121, June 2018, Pages 169-175
نویسندگان
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