کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7705134 | 1496911 | 2015 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Amperometric inhibition biosensors based on horseradish peroxidase and gold sononanoparticles immobilized onto different electrodes for cyanide measurements
ترجمه فارسی عنوان
بیوسنسورهای مهار آمپرمتریک براساس پراکسیداز هورس و پراکسیدازهای طلایی که بر روی الکترودهای مختلف برای اندازه گیری سیانید متصل شده اند
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
الکتروشیمی
چکیده انگلیسی
New biosensors based on inhibition for the detection of cyanide and the comparison of the analytical performances of nine enzyme biosensor designs by using three different electrodes: Sonogel-Carbon, glassy carbon and gold electrodes were discussed. Three different horseradish peroxidase immobilization procedures with and without gold sononanoparticles were studied. The amperometric measurements were performed at an applied potential of â 0.15 V vs. Ag/AgCl in 50 mM sodium acetate buffer solution pH = 5.0. The apparent kinetic parameters (Kmapp, Vmaxapp) of immobilized HRP were calculated in the absence of inhibitor (cyanide) by using caffeic acid, hydroquinone, and catechol as substrates. The presence of gold sononanoparticles enhanced the electron transfer reaction and improved the analytical performance of the biosensors. The HRP kinetic interactions reveal non-competitive binding of cyanide with an apparent inhibition constant (Ki) of 2.7 μM and I50 of 1.3 μM. The determination of cyanide can be achieved in a dynamic range of 0.1-58.6 μM with a detection limit of 0.03 μM which is lower than those reported by previous studies. Hence this biosensing methodology can be used as a new promising approach for detecting cyanide.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 101, February 2015, Pages 84-91
Journal: Bioelectrochemistry - Volume 101, February 2015, Pages 84-91
نویسندگان
Aisha Attar, Laura Cubillana-Aguilera, Ignacio Naranjo-RodrÃguez, José Luis Hidalgo-Hidalgo de Cisneros, José MarÃa Palacios-Santander, Aziz Amine,