کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7769157 1500415 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isolation and characterization of microsatellite loci for the analysis of genetic diversity in Whitmania pigra
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Isolation and characterization of microsatellite loci for the analysis of genetic diversity in Whitmania pigra
چکیده انگلیسی
The objective of this study was to isolate microsatellite loci to analyze the genetic diversity of Whitmania pigra. Four new microsatellite markers of W. pigra were developed from an enriched library and ten from a modified SAMPL assay. A total of 127 alleles were detected, with an average of 9.1 alleles per locus. The expected heterozygosity (He) of each microsatellite locus varied from 0.451 to 0.857, with an average of 0.688. The polymorphism information content (PIC) of each microsatellite locus ranged from 0.361 to 0.838, with an average of 0.640. Analysis of molecular variance showed that the main variation component existed within the populations (81.64%) rather than among the populations (18.36%). Phylogenetic tree for 15 populations of Hirudo using the NJ method by MEGA 5.1 software were divided into two major clusters. These microsatellite markers will contribute to research on the individual identification, genetic diversity, population structure, genome mapping and conservation biology of Hirudo.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Systematics and Ecology - Volume 51, December 2013, Pages 207-214
نویسندگان
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