Optimization and in vitro toxicity evaluation of G4 PAMAM dendrimer-risperidone complexes

► Incorporation of Risp molecules into DG4 was studied. ► The best conditions encountered for drug incorporation were: low solvent polarity mixture, pH c.a. 7, room temperature and low ionic strength. ► DG4-Risp showed no cell viability reduction at concentrations below 3 × 10−2 μM DG4 and 5.1 μM Risp. ► No significant hemolysis or morphological changes were observed on red blood cells. ► Complex resulted structurally stable for a period of 106 h in buffer.