کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7941 570 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Non-destructive label-free monitoring of collagen gel remodeling using optical coherence tomography
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Non-destructive label-free monitoring of collagen gel remodeling using optical coherence tomography
چکیده انگلیسی

Matrix remodeling plays a fundamental role in physiological and pathological processes, as well as in tissue engineering applications. In this paper, optical coherence tomography (OCT), a non-destructive optical imaging technology, was used to image collagen gel remodeling by smooth muscle cells (SMCs). The optical scattering properties of collagen–SMC gels were characterized quantitatively by fitting OCT data to a theoretical model. Matrix remodeling over 5 days produced a 10-fold increase in the reflectivity of the collagen gels, corresponding to a decrease in scattering anisotropy from 0.91 to 0.46. The increase in reflectivity was corroborated in confocal mosaic images. Blocking matrix degradation in collagen–SMC gels with doxycycline, a non-specific matrix metalloproteinases (MMPs) inhibitor, impeded the decrease in scattering anisotropy and resulted in few macroscopic signs of remodeling. Causing matrix degradation in acellular gels with a 3 h treatment of MMP-8 (collagenase 2) partially mimicked the decrease in anisotropy measured in collagen–SMC gels after 5 days. These results suggest that the decrease in scattering anisotropy in the collagen–SMC gels was due to MMP activity that degrades collagen fibrils into smaller fragments.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 31, Issue 32, November 2010, Pages 8210–8217
نویسندگان
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